Year: 2022 | Month: December | Volume 12 | Issue 6
In-vitro Assessment of Antimicrobial Activity Against Isolates and Pure Culture of Salmonella Typhimurium by Using Green Synthesized Silver Nanoparticles
Raghupathi Ch
Vijaya Kumar
A.
Sujatha Singh
Shirisha
A.
Naveena
A.
DOI:10.30954/2277-940X.06.2022.2
Abstract:
The green synthesis of metal nanoparticles has gained significant research attention in recent years. Among metallic nanoparticles, silver nanoparticles (AgNPs) exhibit a broad range of physical, chemical and biological properties due to which they are extensively applied in different fields. In this study, 1mM silver nitrate solution was combined with leaf extracts of Ocimum sanctum and Azadirachta indica under various circumstances to create green silver nanoparticles. Due to the presence of terpenoids and flavonoids, these two plant leaf extracts function as reducing agents in the synthesis of silver nanoparticles. This study discusses produced silver nanoparticles that were examined using a UV-visible spectrophotometer and the Zeta potential method. The absorption spectrum of the silver nano solution prepared by using Ocimum sanctum and Azadirachta indica leaf extracts showed a surface plasmon absorption band with maximum of 440 nm and 410 nm respectively. The zeta value of silver nanoparticles synthesized from Ocimum sanctum and Azadirachta indica leaf extract was -15.3 and -21.5 mV, size of 71.4 nm and 100.6 nm respectively. The antimicrobial activity of silver nanoparticles was compared in the current study using the agar well diffusion method on Salmonella Typhimurium isolates and pure cultures, as well as the anti-microbial resistance of different antibiotics on isolates and pure cultures. Final results demonstrated that silver nanoparticles exhibit antimicrobial activity on Salmonella Typhimurium bacteria that is nearly comparable to that of some antibiotics, and that a green synthesis protocol is an alternative to conventional physical and chemical methods that is quick, eco-friendly, and nontoxic.
Highlights
- Observed the antibacterial activity of silver nano particles in comparison with selected antibiotic discs on Salmonella Typhimurium.
- Green synthesized silver nanoparticles show promising results that can effectively replace antibiotics.
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